Project Summary

Effects of Extended Post-mortem Aging Periods on the Shelf-life and Eating Qualities of Beef Intended for Retail Sale

Principle Investigator(s):
D. R. Woerner, Ph.D.
Institution(s):
Colorado State University
Completion Date:
May 2013
Background 

Beef retailers are seemingly hesitant to age beef for more than 14 days prior to placing it in the retail case because product “freshness” is reduced, and a shorter display life may be realized.  

A more definite understanding of the effects of postmortem aging on the retail display life of USDA Commodity Choice and Select cuts that are most likely to appear in retail supermarkets could lead to a more extensive implementation of aging beef products destined for retail and ultimately decrease the incidence of unacceptably tough steaks sold in retail while optimizing tenderness without sacrificing time in retail display. This is especially relevant with the world’s largest retailer, Wal-Mart, improving the quality of the beef products that they are selling and accompanying them with a “guaranteed satisfaction, money-back” claim. With the ability to confidently utilize extended aging periods to ensure tenderness, companies supplying beef to retailers should experience fewer claims and account for greater customer satisfaction with beef.  

Providing clear evidence of the effects of postmortem aging on the display life and tenderness of beef retail steaks destined for retail supermarkets is essential for retailers to understand their opportunities for improving the marketability and palatability of their beef products. Therefore, this research was intended to clarify the effects of extend postmortem aging periods on beef in the retail sector to provide a recommended aging time for strip loins and sirloin steaks.  

Methodology 

In order to mimic beef commonly found in retail supermarkets, paired strip loins and top sirloin butts (from both the left and right sides of the carcass) were obtained from USDA Choice carcasses with a marbling score ranging from Small00 to Small50 (n = 15) and USDA Select carcasses with a marbling score ranging from Slight50 to Slight99 (n = 15) at a commercial packing plant. Strip loins and top sirloin butts (both the left and right sides) from an individual animal were fabricated into six, 4- inch portions. Each of the 6 portions was randomly assigned to one of 6 postmortem aging periods (14, 21, 28, 35, 49 or 63 days). For both strip loin and sirloin sections, once the aging period was designated, the sections were stored in a vacuum-sealed bag at 0°C (± 1°C) until their assigned aging period were complete. Then, sections were removed from storage and each section was faced and 3, 1-inch steaks were cut from each section. One steak was immediately designated for Warner- Bratzler shear force (WBSF), while the other two steaks was placed in a traditional PVC overwrapped package and placed in a multi-deck retail display case for 72 hours. During the display period, each steak was evaluated every 8 hours by a minimum of 8 trained panelists for lean color (bright cherry-red to very dark red or brown/green), external fat color (bright, creamy white to dark tan or brown/green), and lean percent discoloration (0 to 100%). Immediately following subjective color analysis, L* a* b* lean color values were measured using a spectrophotometer. Following removal, 1 of the 2 steaks was designated for shear force testing and oxidation analysis (TBARS); the other steak was rated by a trained sensory panel for myofibrillar tenderness, connective tissue amount, juiciness, beef flavor intensity, buttery beef fat flavor, oxidized, sour/acidic, livery/organy, and metallic/bloody. 

Findings 

As steaks were subjected to longer periods of postmortem aging, WBSF values decreased and trained sensory panel tenderness ratings improved. A 72-h display time was shown to significantly reduce (P < 0.0001) WBSF values of strip loin and sirloin steaks. A minimum of 28 d of postmortem aging is required to improve the WBSF values of low Choice and Select strip loin steaks over the same strip loins steaks that have been aged for only 14 d, and a minimum of 35 d of postmortem aging is required to significantly improve sensory tenderness ratings for low Choice and Select strip loin steaks. Sensory panel ratings of off-flavors also supported that aging strip loin steaks for 35 d or more postmortem for retail display and sale is undesirable as the intensity of oxidized and sour/acidic off-flavors significantly increased. This data suggests that strip loin steaks can be aged for 28 d prior to retail display with little effect on display life and the incidence of off-flavors, however, there was no significant tenderness advantage over 14 d aged steaks from low Choice and Select strip loins  

The findings for sirloin steaks indicate that 35 d of postmortem aging are required to achieve an improvement in WBSF over 14 d aged low Choice and Select top sirloin steaks, and trained sensory panel scores indicated that at least 49 d of postmortem aging is required to improve the myofibrillar tenderness of low Choice and Select sirloin steaks. Lean color scores indicated a drastically reduced display life for sirloin steaks aged 35 d or more prior to retail display. Sirloin steaks aged 35 d and more produced undesirable lean color scores in as early as the first 24 h of retail display, which would suggest the need to discount the price of these steaks or even remove them from retail display. Additionally, low Choice and Select top sirloin steaks aged only 14 d and displayed an additional 72 h had relatively intense levels of oxidized and sour/acidic flavors present. With all factors considered for low Choice and Select top sirloins, it is not realistic to conclude that top sirloins can be aged for a long enough period to improve tenderness and maintain a considerable level of display life. Long aging treatments for top sirloins is most well suited for foodservice where display life is a non-issue. 

Implications 

Providing clear evidence of the effects of postmortem aging on the display life and tenderness of beef retail steaks destined for retail supermarkets is essential for retailers to understand their opportunities for improving the marketability and palatability of their beef products. Results of this study will provide guidelines for retail display life which could lead to ensuring that consumers can have confidence that they are buying a consistently high-quality product at retail stores. 

Table 1. Trained sensory panel ratings1 for aged strip loin steaks that were in retail display for 72 h.

 

Sensory Attribute

Age (d)

Myofibrillar Tenderness

Con. Tissue Tenderness

Overall Tenderness

Juiciness

Beef Flavor

Buttery/Beef Fat Flavor

Sour /Acidic

Oxidized

Metallic /Bloody

Livery /Organy

14d

88.76

96.09b

98.17b

80.03a

83.09

58.09

8.36b

15.42

4.06

1.75c

21d

86.34C

98.76b

89.84b

75.84bc

84.03

60.45

7.79b

17.23bc

2.09

0.66c

28d

87.60c

97.45b

89.69b

73.30C

84.47

57.36

8.4b

17.82bc

2

1.17c

35d

93.78b

105.6a

99.56a

77.35ab

82.09

59.61

11.1b

21.71bc

3.09

1.5b

49d

96.73ab

105.13a

98.12a

78.86ab

84.24

59.72

16.73a

22.22b

3.7

1.8b

63d

98.74a

108.87a

101.51a.

76.82abc

81.96

56.53

22.13a

31.42a

1.68

3.84a

SEM

1.6

1.46

1.55

1.38

1 .17

1.26

1

2.37

0.52

0.29

Page

<.0001

<.001

<.0001

0.0163

0.4925

0. 1899

<.0001

<.0001

0.0629

0.0079

  • 1 Panelists marked a fixed 150mm line scale to indicate their response. 150mm=highest level of each attribute. 
  • a-e Within column, means without a common superscript letter differ (P<0.05)

Table 2. Trained sensory panel ratings1 for aged sirloin steaks that were in retail display for 72 h.

 

Sensory Attribute

Age (d)

Myofibrillar Tenderness

Con. Tissue Tenderness

Overall Tenderness

Juiciness

Beef Flavor

Buttery/Beef Fat Flavor

Sour /Acidic

Oxidized

Metallic /Bloody

Livery /Organy

14d

82.31b

89.11c

85.95

75.93ab

80.32a

47.97

15.97c

31.42b

4.7

2.52d

21d

85.25b

95.64b

87.53

84.25a

79.99a

47.72

16.04c

32.81b

5.66

5.7bc

28d

85.4b

97.81ab

88.31

73.43bc

79.29a

46.84

17.37c

36.5ab

5.3

4.42cd

35d

85.37b

94.87b

91.3

74.12abc

79.54a

47.67

18.25c

37.52ab

5.77

7.72ab

49d

86.41b

95.98b

88.3

72.18bc

78.36ab

46.34

23.69b

39.95a

5.72

7.04abc

63d

91.57a

101.65a

93.51

71.53c

75.87b

45.65

32.97a

41.39a

7.63

9.85a

SEM

1.76

2.00

1.57

1.13

1.02

1.96

1.76

2.32

0.68

0.62

Page

0.0147

0.0015

0.1006

0.0255

0.0300

0.5517

< 0.0001

0.0187

0.4544

<.0001

  • 1 Panelists marked a fixed 150mm line scale to indicate their response. 150mm=highest level of each attribute. 
  • a-e Within column, means without a common superscript letter differ (P<0.05)

Table 3. Trained sensory panel lean color scores1 for strip loins steaks displayed for 72 h (PAGExHOUR<0.0001).

 

Age (d)

Display (h)

14d

21d

28d

35d

49d

63d

SEM

0

110.49az

95.4cy

89.39abx

84.24dw

84.58cdxw

95.10ay

0.72

8

107.62az

98.04by

94.29ay

95.36by

96.71ay

93.94 ay

0.63

16

109.87az

102.2ay

92.00ax

98.99ay

93.64abx

90.34bx

0.72

24

103.64bz

100.43az

92.84ay

87.07dx

86.91cyx

86.91bx

0.72

32

106.57abz

97.34by

90.89ax

91.23cx

84.25dw

89.28bxw

0.81

40

99.00cz

96.54bzy

81.43dv

86.37dwv

92.48byx

89.39bxw

0.8

48

101.97bz

94.87cy

86.95bxw

82.94dwv

78.79ev

89.22byz

0.89

56

106.45bcz

96.9cy

85.12bcx

88.26dx

84.25dx

87.68bx

0.89

64

105.01 bz

98.41ay

80.68dx

86.26dx

94.19ay

80.99cx

0.88

72

100.72cz

96.5bz

84.89cyx

87.28dy

86.24cy

80.46dx

0.84

SEM

1.59

1.59

1.59

1.59

1.59

1.59

 

  • 1 Panelists marked a fixed 150 mm line scale to indicate their response. Higher values indicate a more bright, cherry-red color while lower values indicate a more dark, purplish red lean color. 
  • a-e Within column, means without a common letter superscript differ (P<0.05) 
  • w-z Within rows, means with a common letter superscript differ (p<0.05)

Table 4. Trained sensory panel lean color scores1 for sirloin steaks (PAGExHOUR<0.0001).

 

Age (d)

Display (h)

14d

21d

28d

35d

49d

63d

SEM

0

109.64az

94.01ay

87.20bx

80.37bw

84.2bx

92.29ay

0.55

8

103.49bz

94.13ay

91.63ay

86.84axw

90.58ayx

85.11bw

0.58

16

105.97bz

92.76ay

90.83ay

85.96ax

84.59bx

80.11cw

0.61

24

93.66cz

88.21by

90.24azy

77.21bx

79.76cx

75.97dx

0.59

32

95.70cz

80.97cx

86.96by

78.27bx

72.09ew

76.97dx

0.63

40

87.53dz

82.85czy

75.89dex

74.60cx

80.84bey

73.85dex

0.74

48

89.54dz

77.05dyx

80.85cy

66.89fw

64.15gw

73.66ex

0.86

56

88.42dz

78.22dy

79.30cy

68.99exw

65.05gw

71.48efx

0.73

64

88.04dz

79.37cdy

75.96dyx

73.92cx

75.41dyx

63.15gw

0.72

72

88.14dz

77.64dy

77.34dy

69.61dx

67.70fx

60.64gw

0.79

SEM

1.32

1.31

1.31

1.31

1.28

1.28

 

  • 1 Panelists marked a fixed 150 mm line scale to indicate their response. Higher values indicate a more bright, cherry-red color while lower values indicate a more dark, purplish red lean color. 
  • a-e Within column, means without a common letter superscript differ (P<0.05) 
  • w-z Within rows, means with a common letter superscript differ (p<0.05)
Photos